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KMID : 1101420200520040381
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Korean Journal of Clinical Laboratory Science
2020 Volume.52 No. 4 p.381 ~ p.388
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Pathological Factors Affecting DNA Quality in BRAF, EGFR, and KRAS Gene Molecular Tests
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Yun Hyon-Goo
Kim Bo-Ra
Lee Joo-Mi
Song Eun-Ha
Kim Dong-Hoon
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Abstract
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The quality control of pathological specimens is important for accurate molecular pathology testing.
This study evaluated that specimen factors affecting the DNA quality during tissue processing and sample types for BRAF, EGFR, and KRAS mutations tests. One thousand seven hundred and seventy-two molecular pathology tests were investigated for the factors influencing the DNA quality, such as sample type, formalin fixation time, and reexamination status. Cytology samples stored in a saline solution had better DNA quality than commercial cytology preservation. Tissue samples fixed in formalin within 24 hours had better DNA quality than the samples fixed over 24 hours. Between the types of samples, fresh tissue samples and tissue samples with a high tumor cell density had relatively better DNA quality than the formalin-fixed paraffin-embedded (FFPE) tissues and cytology specimens. Of real-time PCR, the non-PNA Ct value increased proportionally with samples held for longer than 24 hours in formalin, and that the formalin-fixed time affects the sample DNA quality. In conclusion, the appropriate tumor cellularity and 10% neutral formalin fixation time are the most important factors for maintaining the DNA quality. These factors should be managed properly for an accurate pathological molecular test to ensure optimal DNA quality.
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KEYWORD
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DNA, Formalin fixation, Molecular test, Quality control
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